Neuronal network activity and connectivity are impaired in a conditional knockout mouse model with PCDH19 mosaic expression

Autor: Giansante, Giorgia, Mazzoleni, Sara, Zippo, Antonio G., Ponzoni, Luisa, Ghilardi, Anna, Maiellano, Greta, Lewerissa, Elly, van Hugte, Eline, Nadif Kasri, Nael, Francolini, Maura, Sala, Mariaelvina, Murru, Luca, Bassani, Silvia, Passafaro, Maria
Zdroj: Molecular Psychiatry; June 2024, Vol. 29 Issue: 6 p1710-1725, 16p
Abstrakt: Mutations in PCDH19gene, which encodes protocadherin-19 (PCDH19), cause Developmental and Epileptic Encephalopathy 9 (DEE9). Heterogeneous loss of PCDH19 expression in neurons is considered a key determinant of the disorder; however, how PCDH19 mosaic expression affects neuronal network activity and circuits is largely unclear. Here, we show that the hippocampus of Pcdh19mosaic mice is characterized by structural and functional synaptic defects and by the presence of PCDH19-negative hyperexcitable neurons. Furthermore, global reduction of network firing rate and increased neuronal synchronization have been observed in different limbic system areas. Finally, network activity analysis in freely behaving mice revealed a decrease in excitatory/inhibitory ratio and functional hyperconnectivity within the limbic system of Pcdh19mosaic mice. Altogether, these results indicate that altered PCDH19 expression profoundly affects circuit wiring and functioning, and provide new key to interpret DEE9 pathogenesis.
Databáze: Supplemental Index
Externí odkaz: