| Autor: |
Bash, Judy, Zong, Wei-Xing, Gélinas, Céline |
| Zdroj: |
Molecular and Cellular Biology; November 1997, Vol. 17 Issue: 11 p6526-6536, 11p |
| Abstrakt: |
A tetracycline-regulated system was used to characterize the effects of c-Rel on cell proliferation. The expression of c-Rel in HeLa cells led to growth arrest at the G1/S-phase transition, which correlated with its nuclear localization and the induction of endogenous IκBα expression. These changes were accompanied by a decrease in E2F DNA binding and the accumulation of the hypophosphorylated form of Rb. In vitro kinase assays showed a reduction in Cdk2 kinase activity that correlated with elevated levels of p21WAF1Cdk inhibitor and p53 tumor suppressor protein. While the steady-state levels of WAF1transcripts were increased, pulse-chase analysis revealed a sharp increase in p53 protein stability. Importantly, the deletion of the C-terminal transactivation domains of c-Rel abolished these effects. Together, these studies demonstrate that c-Rel can affect cell cycle control and suggest the involvement of the p21WAF1and p53 cell cycle regulators. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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