| Autor: |
Schaeffer, Michael, Schneiderbauer, Michaela, Weidler, Sascha, Tavares, Rosário, Warmuth, Markus, de Vos, Gabriele, Hallek, Michael |
| Zdroj: |
Molecular and Cellular Biology; December 2001, Vol. 21 Issue: 23 p8068-8081, 14p |
| Abstrakt: |
Interleukin-6 (IL-6) induces the activation of the Src family kinase Hck, which is associated with the IL-6 receptor β-chain, gp130. Here we describe the identification of an “acidic” domain comprising amino acids 771 to 811 of gp130 as a binding region for Hck, which mediates proliferative signaling. The deletion of this region of gp130 (i.e., in deletion mutant d771-811) resulted in a significant reduction of Hck kinase activity and cell proliferation upon stimulation of gp130 compared to wild-type gp130. In addition, d771-811 disrupted the growth factor-stimulated activation of Erk and the dephosphorylation of Pyk2. Based on these findings, we propose a novel, acidic domain of gp130, which is responsible for the activation of Hck, Erk, and Pyk2 and signals cell proliferation upon growth factor stimulation. |
| Databáze: |
Supplemental Index |
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