| Autor: |
KLESIEWICZ, KAROLINA, MROWIEC, PAULINA, KANIA, KATARZYNA, SKIBA-KUREK, IWONA, BIAŁECKA, JOANNA, NAMYSŁ, MAGDALENA, MAŁEK, MARIANNA |
| Zdroj: |
Polish Journal of Microbiology; March 2023, Vol. 72 Issue: 1 p69-77, 9p |
| Abstrakt: |
Candida albicansremains the most common species isolated from women with vulvovaginal candidiasis. However, closely related species such as Candida africanaand Candida dubliniensismay also occur, although they are often misidentified. The aim of the study was to confirm the phenotypic identification of C. albicansand its closely related species isolated from women with genital tract infections by amplification of the hwp1 (hyphal wall protein 1) gene in a PCR assay. We report a detailed molecular identification of C. albicansand its closely related species among 326 patients in the Małopolska region, Poland. Initial phenotypic identifications were confirmed by amplification of the hwp1 gene. Based on molecular analysis, we revealed 307 strains (94.17%) as C. albicansand 17 as C. dubliniensis(5.22%). No strain of C. africanawas detected. Two patients h ad co-infection with C. albicansand C. dubliniensis(0.61%). A PCR assay targeting the hwp1 gene was reliable for correctly identifying species among the C. albicanscomplex. |
| Databáze: |
Supplemental Index |
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