| Autor: |
Cazet, Jack F., Siebert, Stefan, Little, Hannah Morris, Bertemes, Philip, Primack, Abby S., Ladurner, Peter, Achrainer, Matthias, Fredriksen, Mark T., Moreland, R. Travis, Singh, Sumeeta, Zhang, Suiyuan, Wolfsberg, Tyra G., Schnitzler, Christine E., Baxevanis, Andreas D., Simakov, Oleg, Hobmayer, Bert, Juliano, Celina E. |
| Zdroj: |
Genome Research; 2023, Vol. 33 Issue: 2 p283-298, 16p |
| Abstrakt: |
The epithelial and interstitial stem cells of the freshwater polyp Hydraare the best-characterized stem cell systems in any cnidarian, providing valuable insight into cell type evolution and the origin of stemness in animals. However, little is known about the transcriptional regulatory mechanisms that determine how these stem cells are maintained and how they give rise to their diverse differentiated progeny. To address such questions, a thorough understanding of transcriptional regulation in Hydrais needed. To this end, we generated extensive new resources for characterizing transcriptional regulation in Hydra, including new genome assemblies for Hydra oligactisand the AEP strain of Hydra vulgaris, an updated whole-animal single-cell RNA-seq atlas, and genome-wide maps of chromatin interactions, chromatin accessibility, sequence conservation, and histone modifications. These data revealed the existence of large kilobase-scale chromatin interaction domains in the Hydragenome that contain transcriptionally coregulated genes. We also uncovered the transcriptomic profiles of two previously molecularly uncharacterized cell types: isorhiza-type nematocytes and somatic gonad ectoderm. Finally, we identified novel candidate regulators of cell type–specific transcription, several of which have likely been conserved at least since the divergence of Hydraand the jellyfish Clytia hemisphaericamore than 400 million years ago. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
