| Autor: |
Chandrababu, Krishnapriya, Sreelatha, Harikrishnan Vijayakumar, Sudhadevi, Tara, Anil, Arya, Arumugam, Sabareeswaran, Krishnan, Lissy Kalliyana |
| Zdroj: |
The Journal of Spinal Cord Medicine; March 2023, Vol. 46 Issue: 2 p262-276, 15p |
| Abstrakt: |
Background:The multipotency of adipose-derived mesenchymal stem cells (ADMSC) could be an advantage to regenerate tissues with multiple cell types. However, due to the hostile nature, trauma sites like spinal cord injury can augment the ADMSC differentiation into undesirable lineages. Immersing pre-differentiated neural progenitors in a biomimetic niche during delivery could guard them against any undesired differentiation or death.Objective:The study proposes using an insoluble cell-specific fibrin niche for in vitrodifferentiation of rat ADMSCs to neural progenitor cells (NPCs) and oligodendrocyte progenitor cells (OPCs). Further, the study explores fibrin hydrogel for in vivoprogenitor cell delivery, and that can aid post-transplant survival/differentiation.Design:The in vitroexperiments analyzed for differentiation-specific markers to establish derivation of rADMSCs to rNPCs and rOPCs. The derived progenitors, tagged with fluorescent tracker dye were delivered in rat T10 contusion SCI using fibrin hydrogel. After 28 days, imaged the experiment site to determine cell survival, immunostained the tissues to identify differentiation of transplanted cells, and evaluated the effect of fibrin and cells on regulating the injury-associated immune response.Results:The study demonstrated fibrin niche aided stable differentiation of rat ADMSCs into neural progenitors. Fibrin matrix holds up the delivered progenitor cells in the SCI site. The H&E stained tissues revealed regulated cavitation, astrogliosis, and inflammation in test tissues. Progression of transplanted cells into oligodendrocytes upon delivering a mixture of rNPCs, rOPCs, and fibrin is evident.Conclusion:Fibrin niche-based derivation of neural progenitors from ADMSC seems valuable for transplantation using fibrin hydrogel. It is a promising strategy for extensive study towards further development of translational stem cell-based neural replacement therapy. |
| Databáze: |
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