| Abstrakt: |
In this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated against Alternaria dauciinfection on carrot (Daucus carotaL.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and TEM) and ultra-violet (UV) visible spectroscopy were used to validate and characterize MgO NPs. The crystalline nature of MgONPs was determined using selected area electron diffraction (SAED). MgO NPs triggered substantial antifungal activity against A. dauciwhen exposed to 50 and 100 mg L−1concentrations but the higher antifungal potential was noticed in 100 mg L−1under in-vitroconditions. In fungal inoculated plants, a marked decrease in growth, photosynthetic pigments, and an increase in phenol, proline contents, and defense-related enzymes of carrot were seen over control (distilled water). However, foliar application of MgO NPs at 50 and 100 mg L−1resulted in significant improvement of plant growth, photosynthetic pigments, phenol and proline contents, and defense enzymes activity of carrots with and without A. dauciinfection. Spraying of MgO NPs at 100 mg L−1had more plant length (17.11%), shoot dry weight (34.38%), plant fresh weight (20.46%), and root dry weight (49.09%) in carrots when challenged with A. dauciover inoculated control. The leaf blight indices and percent disease severity were also reduced in A. dauciinoculated plants when sprayed with MgO NPs. The non-bonding interactions of Alternariagenus protein with nanoparticles were studied using molecular docking. |
Full Text from ScienceDirect