| Autor: |
Evans, Robert John, Bauer, Doris H., Flegal, Cal J. |
| Zdroj: |
The Journal of Nutrition; March 1971, Vol. 101 Issue: 3 p355-361, 7p |
| Abstrakt: |
Lipovitellins were isolated from normal eggs and from eggs laid by hens fed crude cottonseed oil (“cottonseed” lipovitellin). Cottonseed lipovitellin contained 23% lipid compared to 17% in normal lipovitellin, and the lipids of cottonseed lipovitellin contained more stearic acid and less oleic acid than those of normal lipovitellin. Amino acid compositions of the vitellins (proteins) were similar. Ether extracted 49% of the lipid from cottonseed lipovitellin compared to 16% of the lipid from normal lipovitellin. Progressive digestion with proteolytic enzymes released similar amounts of lipids and peptides from ether-extracted normal and cottonseed lipovitellins. Chromatography on a TEAE-cellulose column separated lipovitellins into α- and β-lipovitellins. Normal lipovitellins were better separated than cottonseed lipovitellins, and the ratio of α-lipovitellin to β-lipovitellin was smaller in normal than in cottonseed lipovitellins. The principal difference between normal and cottonseed lipovitellins appeared to be in the higher content of saturated fatty acids in the lipid, the larger amount of lipid bound on the outer surface of the molecule, and the higher proportion of α-lipovitellin in cottonseed lipovitellins than in normal lipovitellins. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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