| Abstrakt: |
Methicillin-susceptible Staphylococcus aureus(MSSA) is an important nosocomial pathogen worldwide. This study aims to investigate the in vitro biofilm-forming ability of clinical MSSA isolated from various sources in the main public tertiary referral hospital in Terengganu, Malaysia and to detect the presence of biofilm-associated and regulatory genes among these isolates. A total of 104 MSSA isolates [pus (n= 75), blood (n= 24), respiratory secretions (n= 2), eye (n= 2), and urine (n= 1)] were investigated for slime production and biofilm formation using Congo red agar and crystal violet microtitre plate, respectively. Fifteen MSSA isolates with varying degrees of biofilm formation were selected for validation via a real-time cell analyser. All isolates were screened for microbial surface components recognising adhesive matrix molecules (MSCRAMM) and accessory gene regulator (agr) using polymerase chain reaction assay. A total of 76.0% (79/104) isolates produced slime layer, while all isolates developed biofilm as follows: 52.8% (55/104) strong biofilm producers, 40.4% (42/104) intermediate biofilm producers, and 6.7% (7/104) weak biofilm producers. A total of 98.1% (102/104) isolates carried at least one of the screened MSCRAMM gene(s) with the enogene detected at the highest rate (87.5%, 91/104), while the sasGgene was significantly associated with strong biofilm production (p= 0.015). Three agrgroups, 1, 2, and 3, were detected among the MSSA isolates with a predominance of agr-3(32.7%, 34/104). In conclusion, biofilm formation varied greatly among clinical MSSA isolates, and the presence of sasGgene and agr-1may play important role in initiating MSSA infections via biofilm formation. |
Full text from SpringerLink