| Autor: |
Sajanti, Antti, Hellström, Santtu, Girard, Romuald, Frantzen, Janek, Umemori, Juzoh, Koskimäki, Fredrika, Lyne, Sean B., Falter, Johannes, Rantamäki, Tomi, Nazir, Kenneth, Gharat, Bajera, Posti, Jussi, Roine, Susanna, Takala, Riikka, Kolehmainen, Sulo, Cao, Ying, Li, Yan, Heino, Iiro, Rahi, Melissa, Rinne, Jaakko, Nieminen, Anni I, Castren, Eero, Koskimaki, Janne |
| Zdroj: |
Stroke (Ovid); February 2023, Vol. 54 Issue: Supplement 1 pATP234-ATP234, 1p |
| Abstrakt: |
Background:Neuronal recovering processes after acute brain injuries such as aneurysmal subarachnoid hemorrhage (aSAH), ischemic stroke (IS) and traumatic brain injury (TBI) are complex including brain plasticity and synaptic regeneration mechanisms. Temporal changes in lipidomic profile after different acute brain injuries may reveal new insights about brain plasticity, recovery and biomarker development after acute injury.Hypotheses:Measurable temporal changes in serum lipidomic profile may be similar irrespective of the type of the brain injury and may reveal conserved molecular aberrations after different types of acute brain injuries. These common changes may also be associated to outcome and serve as possible biomarkers.Methods:Prospective cohort (n=74) consisted of IS (n=30), aSAH (n=31) and TBI (n=13) patients. Serum samples were collected in two time points after the insult (early 24-48h and late 120-192h). Lipidomic profiling of 1153 lipids was performed using Lipidyzer Platform. The lipidomics data was analyzed with Metaboanalyst for ANOVA, PCA, heatmap, box plots with following settings: missing values were imputated with KNN using 20% cutoff, log-transformed and auto-scaled.Results:We identified four diacylglycerols (DAG), one cholesterol ester (CE), seven triacylglycerols (TAG) and one sphingomyelin (SM) which showed increased levels in late serum samples across all types of brain injuries (p<0.05, FDR corrected). Four free fatty acids (FFA) and two phosphatidylethanolamines (PE) levels were decreased in late serum samples across all injury types (p<0.05, FDR corrected).Conclusion:Changes in lipidomic profiles across time were identified. This suggests common lipidomic characteristics irrespective of the type of brain insult and encourage further studies. Interestingly, lipidomic profiles may be associated to recovery after acute brain injury and further analyses of this data will follow. |
| Databáze: |
Supplemental Index |
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