| Autor: |
WANG, Xiao-dong, CAI, Ying, PANG, Cheng-ke, ZHAO, Xiao-zhen, SHI, Rui, LIU, Hong-fang, CHEN, Feng, ZHANG, Wei, FU, San-xiong, HU, Mao-long, HUA, Wei, ZHENG, Ming, ZHANG, Jie-fu |
| Zdroj: |
Journal of Integrative Agriculture; 20230101, Issue: Preprints |
| Abstrakt: |
Plant height is a key plant architectural trait that affects seed yield, harvest index and lodging resistance in Brassica napusL., however, the genetic mechanisms affecting plant height remain elusive. Here, a semi-dwarf mutant, df34, was obtained by ethyl methanesulphonate-mutagenized. First, genetic analysis showed that the semi-dwarf phenotype was controlled by one semi-dominant gene, which was located on chromosome C03 using a bulked segregant analysis coupled with whole-genome sequencing and named BnaSD.C3. Subsequently, BnaSD.C3was fine-mapped to a 297.35-kb segment of the “Darmor-bzh” genome, but there was no potential candidate gene for the semi-dwarf trait underlying this interval. Furthermore, the interval was aligned to the Zhongshuang 11 reference genome. Finally, combining structural variation analysis, transcriptome sequencing, phytohormone analyses and gene annotation information, BnaC03G0466900ZSand BnaC03G0478900ZSwere determined as the most likely candidate genes affecting plant height of df34. This study provided a novel major locus for breeding and new insights into understanding the genetic architecture of plant height in B. napus. |
| Databáze: |
Supplemental Index |
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