| Abstrakt: |
Breast cancer, as a leading cause of death among females, has registered a high rate of incidence throughout the world. Thus, its ablation requires applying novel approaches, like nanomedicine. In this work, gold (Au) nanoparticles-entrapped and folic acid-functionalized PLGA nanocomposites were fabricated for suppression of MCF-7 breast cancer cells applying NIR irradiation (808 nm for 10 min at a distance of 5 cm and power density of 2.5 W/cm2). After synthesizing the FA-PLGA-Au nanocomposites, they were characterized using FT-IR, 1H NMR, DLS, SEM, TEM, and TGA analyses. The successful synthesis of the nanocomposite, the size of 30 nm–55 nm, 1.4 mV surface charge, globular shape, and 1.1% entrapment efficiency of Au were confirmed for FA-PLGA-Au nanocomposites. Next, several biomedical assessments, including cell viability, qRT-PCR, cell cycle arrest, apoptosis, and cellular uptake were carried out on MCF-7 and MSC normal cell lines to evaluate the PTT ability of nanocomposite. A 26% cell viability with IC50of 200 nM was observed after 72 h of treatment FA-PLGA-Au on cancer cells while it was more than 65% for MSC cells. The apoptotic Caspase9, Bax, and P53 genes expressions are raised to 4.3, 6.9, and 7.8 folds respectively and Bcl2 gene expression declined to 0.5 fold after treatment with FA-PLGA-Au. About 18 and 30 folds increase (compared to control) in apoptosis was attained for nanocomposite using flow cytometry assays, and cellular uptake was indicated at 96% for PLGA-FA samples. These data have approved the potential ability FA-PLGA-Au in PTT of cancer. |
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