| Abstrakt: |
Wheat flour has been implicated in several outbreaks of foodborne illness in recent years, yet little information is available regarding microbial pathogens in wheat and wheat flour. Information about microbial pathogens in wheat is needed to develop effective methods to prevent foodborne illnesses caused by wheat products. From 2012 to 2014, we conducted a baseline study to determine the prevalence and levels of pathogens in wheat samples taken before milling. A total of 5,176 wheat samples were tested for enterohemorrhagic Escherichia coli(EHEC), Salmonellaspp., Listeriaspp., and L. monocytogenes. Positive samples were assayed for most probable numbers (MPNs), and isolates were fingerprinted by pulsed-field gel electrophoresis (PFGE). The rate of detection of each pathogen tested was as follows: Salmonellawas in 1.23% of the samples (average level of 0.110 MPN/g), EHECs occurred in 0.44% of the samples (0.039 MPN/g), and Listeriaspp. occurred in 0.08% of samples (0.020 MPN/g), but L. monocytogeneswas not detected. The PFGE assessment found a high diversity for all organisms. All EHEC PFGE patterns (22 of 22) were unique, and 39 of 47 Salmonellapatterns (83%) were unique. These results indicate a diverse background of naturally occurring organisms. These findings suggest that the microbial contamination is coming from diverse sources and provide no evidence in support of a specific pathogen load. Altogether, our surveillance study shows that contamination of wheat with pathogens is clearly evident and poses a foodborne illness risk. |
