| Autor: |
Rhoades, J., Manderson, K., Wells, A., Hotchkiss, A.T., Gibson, G.R., Formentin, K., Beer, M., Rastall, R.A. |
| Zdroj: |
Journal of Food Protection; November 2008, Vol. 71 Issue: 11 p2272-2277, 6p |
| Abstrakt: |
The aim of the study was to investigate the ability of pectic oligosaccharides (POS) to inhibit adhesion of three strains of verotoxigenic Escherichia coli, three strains of enteropathogenic E. coli, and one nonclinical strain of Desulfovibrio desulfuricansto human intestinal epithelial cell cultures. Lactobacillus acidophilusand Lactobacillus gasseriwere included for comparison. Attachment was determined in the human HT29 cell line by viable count of adherent bacteria. POS in buffer at pH 7.2 were antiadhesive at a dose of 2.5 mg ml−1, reducing adhesion of enteropathogenic E. coliand verotoxigenic E. colistrains to less than 30% of control values. Concentrations resulting in 50% inhibition ranged from 0.15 to 0.46 mg ml−1. L. acidophiluswas not significantly affected, but adhesion of L. gasseriwas reduced to 29% of the control value. POS reduced the adhesion of D. desulfuricansto 0.33% of the control value. POS also had a protective effect against E. coliverocytotoxins VT1 and VT2 at concentrations of 0.01 and 1 μgml−1, respectively. |
| Databáze: |
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