Efficient transformation of Marchantia polymorphathat is haploid and has very small genome DNA

Autor:	Nasu, Masao, Tani, Katsuji, Hattori, Chizuko, Honda, Motoyasu, Shimaoka, Taise, Yamaguchi, Nobuyasu, Katoh, Kenji
Zdroj:	Journal of Fermentation and Bioengineering; January 1997, Vol. 84 Issue: 6 p519-523, 5p
Abstrakt:	The genomic DNA content of a cultured cell of Marchantia polymorphaHYA-2F was examined using a flow cytometer. It was estimated to be 0.32 pg (C), with a G+C content of 57.1%. The DNA content was less than that of Arabidopsis thaliana. The frequency of transformation by Agrobacterium tumefaciensusing a binary vector plasmid pBI121 in the presence of acetosyringone was aproximately 10%. GUS expression analysis and Southern blotting analysis of the genomic DNA of transformants revealed that all regions of T-DNA on plasmid pBI121 were integrated into the genome of M. polymorpha.
Databáze:	Supplemental Index
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