| Autor: |
Paquelet, Grace E., Carrion, Kassandra, Lacefield, Clay O., Zhou, Pengcheng, Hen, Rene, Miller, Bradley R. |
| Zdroj: |
STAR Protocols; March 2023, Vol. 4 Issue: 1 |
| Abstrakt: |
In vivobrainstem imaging with miniature microscopy has been challenging due to surgical difficulty, high motion, and correlated activity between neurons. Here, we present a protocol for brainstem imaging in freely moving mice using the dorsal raphe nucleus as an example. We describe surgical procedures to inject a virus encoding GCaMP6m and securely implant a GRIN lens in the brainstem. We then detail motion correction and cell segmentation from the data to parse single-cell activity from correlated networks. |
| Databáze: |
Supplemental Index |
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