| Autor: |
Amy Lyu, Ming-Ju, Tang, Qiming, Wang, Yanjie, Essemine, Jemaa, Chen, Faming, Ni, Xiaoxiang, Chen, Genyun, Zhu, Xin-Guang |
| Zdroj: |
Plant Communications; January 2023, Vol. 4 Issue: 1 |
| Abstrakt: |
C4photosynthesis evolved from ancestral C3photosynthesis by recruiting pre-existing genes to fulfill new functions. The enzymes and transporters required for the C4metabolic pathway have been intensively studied and well documented; however, the transcription factors (TFs) that regulate these C4metabolic genes are not yet well understood. In particular, how the TF regulatory network of C4metabolic genes was rewired during the evolutionary process is unclear. Here, we constructed gene regulatory networks (GRNs) for four closely evolutionarily related species from the genus Flaveria, which represent four different evolutionary stages of C4photosynthesis: C3(F. robusta), type I C3-C4(F. sonorensis), type II C3-C4(F. ramosissima), and C4(F. trinervia). Our results show that more than half of the co-regulatory relationships between TFs and core C4metabolic genes are species specific. The counterparts of the C4genes in C3species were already co-regulated with photosynthesis-related genes, whereas the required TFs for C4photosynthesis were recruited later. The TFs involved in C4photosynthesis were widely recruited in the type I C3-C4species; nevertheless, type II C3-C4species showed a divergent GRN from C4species. In line with these findings, a 13CO2pulse-labeling experiment showed that the CO2initially fixed into C4acid was not directly released to the Calvin–Benson–Bassham cycle in the type II C3-C4species. Therefore, our study uncovered dynamic changes in C4genes and TF co-regulation during the evolutionary process; furthermore, we showed that the metabolic pathway of the type II C3-C4species F. ramosissimarepresents an alternative evolutionary solution to the ammonia imbalance in C3-C4intermediate species. |
| Databáze: |
Supplemental Index |
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