| Autor: |
Santiago-Velasco, Mayra, Ortiz-López, Erick, Flores-Méndez, Alexis, Barrera-Figueroa, Blanca Estela, García-López, Edgar, Peña-Castro, Julián Mario |
| Zdroj: |
All Life; December 2022, Vol. 15 Issue: 1 p942-950, 9p |
| Abstrakt: |
Submergence stress in plants subjected to flooding is a significant issue in agriculture. Decades of previous research have elucidated many molecular and physiological responses of plants to this stress. Since Arabidopsis thalianahas been established as a model organism in this area of research, in this study, we started by replicating published results of seven sensitive and tolerant ecotypes of Arabidopsisto dark-submergence stress to assess its generalizability and consistency, adapted a leaf-damage index to quantify the progression of stress over time, and measured the recalcitrance of all those ecotypes to Agrobacteriumtransformation. By using photographic comparisons, median lethal time (LT50), and leaf-damage index, we ascertained that the order of tolerance reported by previous studies is robust enough to allow independent replications (Cvi-0 < Ita-0 < Bay-0 < Col-0 < Kin-0 < Lp2-6 < C24), supporting their consistency. We continued by testing the transformation recalcitrance of all seven ecotypes to three different Agrobacteriumstrains and identified C24 and Cvi-0 as the most suitable ecotypes useful in a transformation platform despite their low transformation frequencies (<0.1%). The results from this study can be useful in developing transgenic methodologies that enhance plant responses to stress and transitioning these methodologies from the laboratory to the field. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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