| Autor: |
Oo, James A., Pálfi, Katalin, Warwick, Timothy, Wittig, Ilka, Prieto-Garcia, Cristian, Matkovic, Vigor, Tomašković, Ines, Boos, Frederike, Izquierdo Ponce, Judit, Teichmann, Tom, Petriukov, Kirill, Haydar, Shaza, Maegdefessel, Lars, Wu, Zhiyuan, Pham, Minh Duc, Krishnan, Jaya, Baker, Andrew H., Günther, Stefan, Ulrich, Helle D., Dikic, Ivan, Leisegang, Matthias S., Brandes, Ralf P. |
| Zdroj: |
Cell Reports; November 2022, Vol. 41 Issue: 7 |
| Abstrakt: |
In healthy vessels, endothelial cells maintain a stable, differentiated, and growth-arrested phenotype for years. Upon injury, a rapid phenotypic switch facilitates proliferation to restore tissue perfusion. Here we report the identification of the endothelial cell-enriched long non-coding RNA (lncRNA) PCAT19, which contributes to the proliferative switch and acts as a safeguard for the endothelial genome. PCAT19is enriched in confluent, quiescent endothelial cells and binds to the full replication protein A (RPA) complex in a DNA damage- and cell-cycle-related manner. Our results suggest that PCAT19limits the phosphorylation of RPA2, primarily on the serine 33 (S33) residue, and thereby facilitates an appropriate DNA damage response while slowing cell cycle progression. Reduction in PCAT19levels in response to either loss of cell contacts or knockdown promotes endothelial proliferation and angiogenesis. Collectively, PCAT19acts as a dynamic guardian of the endothelial genome and facilitates rapid switching from quiescence to proliferation. |
| Databáze: |
Supplemental Index |
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