| Autor: |
Broft, Patrizia, Rosenkranz, Remus R.E., Schleiff, Enrico, Hengesbach, Martin, Schwalbe, Harald |
| Zdroj: |
RNA Biology; December 2022, Vol. 19 Issue: 1 p266-278, 13p |
| Abstrakt: |
ABSTRACTTemperature-dependent alternative splicing was recently demonstrated for intron 2 of the gene coding for heat shock factor HsfA2 of the tomato plant Solanum lycopersicum, but the molecular mechanism regulating the abundance of such temperature-dependent splice variants is still unknown. We report here on regulatory pre-mRNA structures that could function as regulators by controlling the use of splice sites in a temperature-dependent manner. We investigate pre-mRNA structures at the splice sites of intron 2 of the gene coding for HsfA2 from S. lycopersicumusing NMR- and CD-spectroscopy as well as in-line probing. The pre-mRNA undergoes conformational changes between two different secondary structures at the 3ʹ splice site of the intron in a temperature-dependent manner. Previously, it was shown that three single nucleotide polymorphisms (SNPs) in intron 2 of the HsfA2 pre-mRNA affect the splicing efficiency of its pre-mRNA and are linked to the thermotolerance in different tomato species. By comparing pre-mRNA fragments of the tomato species S. lycopersicumand S. peruvianum, we show that these SNPs result in substantial structural differences between the pre-mRNAs of the two species. |
| Databáze: |
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