| Autor: |
Vergelli, M, Olivotto, J, Castigli, E, Gran, B, Raimondi, L, Pirisino, R, Amaducci, L, Massacesi, L |
| Zdroj: |
Immunopharmacology; October 1997, Vol. 37 Issue: 2-3 p191-197, 7p |
| Abstrakt: |
Pharmacokinetics and pharmacodynamics of 13-cis-retinoic acid (13-cRA) administered at doses that suppress experimental autoimmune encephalomyelitis (EAE) have been investigated in rats. Serum concentration of the drug measured following oral administration of 37 mg/kg/12 h reached a peak of 1.8×10−5M in 2 h and linearly declined to 7.8×10−7M at hour 12. When spleen cells (SC) collected from 13-cRA-administered animals were cultured in vitro, their proliferative response to the T-cell mitogen concanavalin A (ConA) was suppressed and this effect was dependent on in vivo serum concentrations of the drug. In addition, in vitro exposure of antigen-specific T-cell lines to 13-cRA concentrations equivalent to those observed in vivo caused a dose-dependent suppression of the proliferation induced by the antigen as well as by T-cell mitogens. On a molar basis, 13-cRA showed a stronger in vitro immunosuppressive activity than two immunosuppressive agents used in human therapy, cyclosporin A and 6-mercaptopurin. |
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