| Autor: |
Yu, Deshui, Fan, Renchun, Zhang, Ling, Xue, Pengya, Liao, Libing, Hu, Meizhen, Cheng, Yanjun, Li, Jine, Qi, Ting, Jing, Shaojuan, Wang, Qiuyun, Bhatt, Arvind, Shen, Qian-Hua |
| Zdroj: |
The Crop Journal; 20220101, Issue: Preprints |
| Abstrakt: |
Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner. In barley, HvWRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus, Blumeria graminisf. sp. hordei(Bgh). However, the molecular features of HvWRKY2 in its DNA-binding and repressor functions, as well as its target genes, are uncharacterized. We show that the W-box binding of HvWRKY2 requires an intact WRKY domain and an upstream sequence of ∼75 amino acids, and the HvWRKY2 W-box binding activity is linked to its repressor function in disease resistance. Chromatin immunoprecipitation (ChIP)-seq analysis identified HvCEBiP, a putative chitin receptor gene, as a target gene of HvWRKY2 in overexpressing transgenic barley plants. ChIP-qPCR and Electrophoretic Mobility Shift Assay (EMSA) verified the direct binding of HvWRKY2 to a W-box-containing sequence in the HvCEBiPpromoter. HvCEBiP positively regulates resistance against Bghin barley. Our findings suggest that HvWRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern (PAMP) recognition receptor genes, suggesting that HvCEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bghinfection. |
| Databáze: |
Supplemental Index |
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