| Autor: |
Hariharan, K, Rajagopal Rao, D |
| Zdroj: |
Proceedings Mathematical Sciences; January 1978, Vol. 87 Issue: 1 p63-66, 4p |
| Abstrakt: |
Saline extracts of several varieties ofTriticalehad haemagglutinin activity against rabbit, rat and fowl erythrocytes. In contrast to the wheat germ lectin theTriticalelectin was inactive against human B, 0 blood group type erythrocytes and rather high concentrations of the lectin are needed to agglutinate human A blood group type erythrocytes. TheTriticalelectin was purified about 20-fold with a 10% recovery of activity from one of the varieties (DTS 138) by (NH4)2SO4fractionation followed sequentially by chromatography on DEAE-cellulose and sulphopropyl-Sephadex. Approximately 4 μg of the purified lectin caused visible agglutination with trypsinised rabbit erythrocytes. Among a variety of sugars tested D-glucose, D-mannose and N-acetyl-D-glucosamine (2·5-7·5mM) caused inhibition of agglutination. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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