| Autor: |
Dahal, Neelima, Osterberg, Jeffrey A., Braun, Benjamin, Caldwell, Tom P., Divan, Ralu, Harcum, Sarah W., Wang, Pingshan |
| Zdroj: |
IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology; December 2022, Vol. 6 Issue: 4 p566-573, 8p |
| Abstrakt: |
New methods to rapidly detect and identify Candida cells in patients’ blood is needed for proper candidemia treatment. In this work, a tunable microwave interferometer was used to measure single Candida cells of C. albicans, C. tropicalis, C. parapsilosis, and C. krusei at multiple frequencies between 0.265 GHz and 7.76 GHz. The obtained permittivity values Δϵ' and Δϵ” of non-budding Candida were colinear with cell volumes and had a coefficient that depends on measurement frequency, cell species and viability. Viable and non-viable cells had significant permittivity differences at lower frequency spectrum, but with substantial overlap at 7.76 GHz. For single non-budding cells, measurements at 1.32 GHz and 1.85 GHz enabled a minimum 0.875 classification accuracy with quadratic discriminate analysis (QDA). However, cell budding induced significant microwave property overlaps. Further work is needed to achieve better cell species discrimination. Additionally, it was determined that treating these yeast cells with Caspofungin (CSP) diacetate in dimethyl sulfoxide (DMSO) for 10 minutes significantly altered cell microwave properties. This change was likely due to damaged cell wall and membranes, and the level of change was Candida species dependent. Therefore, broadband microwave measurement is a promising new approach for physicians to provide personalized candidemia therapy. |
| Databáze: |
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