| Autor: |
Sun, Feifei, Yan, Chunlei, Jia, Qi, Wu, Wei, Cao, Yindi |
| Zdroj: |
Journal of Analysis and Testing; 20220101, Issue: Preprints p1-10, 10p |
| Abstrakt: |
Diseases caused by foodborne pathogens are one of the main burdens of public health and seriously hinder global social and economic development. In this research, a novel lateral flow strip was successfully constructed for the simultaneous detection of Gram-positive and Gram-negative bacteria based on the fluidity and color aggregation effect of Au nanoparticles (AuNPs). The lateral flow strip first combined ampicillin (AMP) antigens with AuNPs to form AuNPs-AMP antigens as the first recognition molecule. Then vancomycin specifically recognizing Gram-positive bacteria, and aptamers specifically recognizing Gram-negative bacteria were used as the second recognition molecules. Finally, Gram-positive bacteria and Gram-negative bacteria were detected rapidly using this sandwich mode. The strip can rapidly test the samples within 5 min. Using a lateral flow strip detector, the limit of detection was 4 CFU/mL, and the recovery rates in honey samples were 78.2–88.6%. The lateral flow strip constructed can detect foodborne pathogens quickly, accurately and efficiently, which is of great significance for food safety detection. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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