| Abstrakt: |
Ectoine and hydroxyectoine are compatible solutes with enormous potential for use in the medical and cosmetic industries. Considering the excellent osmoprotective properties of these compatible solutes, we investigate the presence of four compatible solutes (ectoine, hydroxyectoine, proline, and glutamic acid) quantitatively by liquid chromatography-tandem mass spectrometry (LC–MS/MS) in forty-five halophilic/halotolerant bacterial isolates. We determined ectoine production by Marinibacillussp., Nesterenkonia xinjiangensis, Halobacillussp., Bacillus patagoniensis, Virgibacillus picturae, Halomonas neptunia, Bacillus patagoniensis, Gracilibacillussp., Thalassobacillus devorans, Microbacteriumsp., Nesterenkoniasp., and Bacillus agaradhaerens, and this production was NaCl dependent. Additionally, the production of hydroxyectoine was observed in six bacterial isolates (Nesterenkonia xinjiangensis, Halobacillussp., Halomonas neptunia, Thalassobacillus devorans, Nesterenkoniasp., and Bacillus agaradhaerens) which was NaCl and temperature dependent. The study identified new bacterial isolates producing ectoine or hydroxyectoine. While the ectoine production in many different Bacillusmembers and a few Nesterenkoniahave been documented before, ectoine production by Bacillus patagoniensisand Nesterenkonia xinjiangensishas not been shown so far. Further, ectoine production by a member of the genus Thalassobacillus(Thalassobacillus devorans) was demonstrated experimentally for the first time. The findings reported in the study may serve as a basis for the large-scale production of ectoine and hydroxyectoine in the future. |
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