| Abstrakt: |
The current work aims to evaluate the growth rate, molecular patterns and biological activities of Arthrospira platensiscultivated in culture enriched with different chelated minerals (Fe, Zn, Cu, Mn, and Mg) of leather protein hydrolysates (LPHs) and glycine in addition to determine the phytochemical contents of Arthrospira platensiscultivated in modified Zarrouk medium. Also, the work study the protein profile with molecular variations (using SDS-PAGE, Comet assay, and SCOT-PCR) in collected biomasses when compared with control (cyanobacteria cultivated in Zarrouk medium). Superoxide dismutase (SOD), glutathione peroxidase (GPX), and mineral content of each treatment were also determined. The obtained Results showed that a significant increment of growth rate of A. platensisin glycinate treatments was observed especially with Cu-glycinate more than control medium. Additionally, T11 has high O.D. than other chelated minerals but control medium had the highest O.D. than LW-chelated minerals. All A. platensistreated with the glycine-chelates possessed both conserved and additional proteins in their SDS-PAGE banding patterns. Moreover, A. platensistreated by chelated minerals with leather waste hydrolysate induced high discrimination than their analogues chelated with glycine. Six ScoT primers were applied and produced 323 amplicons with an average of 68% polymorphism/primer. SDS and Scot dendrogram revealed that among all treatments, control and Mg-glycine-chelated treatment were closely related. All A. platensissamples treated by chelated minerals with leather waste hydrolysate possessed more polymorphic bands than monomorphic ones (either as proteins or DNA), despite that no significant DNA damage (as % tDNA) was detected on A. platensistreated with either chelated treatment. |
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