| Abstrakt: |
Bacteriophages have been mainly used in treating infections caused by planktonic bacterial cells in the veterinary sector. However, their applications as antibiofilm agents have received little attention. Accordingly, a previously isolated Salmonellainfecting Siphoviridaephage was investigated for host range against 15 Salmonella enterica isolates (S. Cape, S. Gallinarum, 4 S. Enteritidis, 3 S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, S. Agona, S. Daula, and S. Aba) recovered from the litters of commercial broiler farms. All S. enterica isolates were examined for their biofilm activity using a microtiter plate assay and for adrA, csgD, and gcpAgenes using conventional PCR. The phage efficacy against established biofilms produced by the selected seven S. entericaisolates (S. Gallinarum, S. Enteritidis, S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, and S. Agona) was assessed using microtiter plate assay and reverse transcriptase real-time PCR over different incubation times of 5 and 24 h. All S. entericaisolates were strong biofilm formers. Moreover, the phage effectively reduced the biofilm activity of the established S. entericabiofilms in the microtiter plate assay using the independent sample t-test (P< 0.050). Furthermore, the relative expression levels of csgD, gcpA, and adrAgenes in the biofilm cells of S. entericaisolate after phage treatment were significantly up-regulated to variable degrees using the independent sample t-test (P< 0.050). In conclusion, the present study revealed the potential use of Salmonellaphage in reducing established biofilms produced by S. entericaserovars isolated from broiler farms. |
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