| Autor: |
Arai, Sakura, Ooka, Tadasuke, Shibata, Mizuha, Nagai, Yuhki, Tokoi, Yuki, Nagaoka, Hiromi, Maeda, Rika, Tsuchiya, Akihiko, Kojima, Yuka, Ohya, Kenji, Ohnishi, Takahiro, Konishi, Noriko, Ohtsuka, Kayoko, Hara-Kudo, Yukiko |
| Zdroj: |
Foodborne Pathogens & Disease; December 2022, Vol. 19 Issue: 12 p823-829, 7p |
| Abstrakt: |
Escherichia albertiiis an emerging enteropathogen. Several foodborne outbreaks of E. albertiihave been reported in Japan; however, foods associated with most outbreaks remain unidentified. Therefore, polymerase chain reaction (PCR) assays detecting E. albertiispecifically and sensitively are required. Primers and probe for real-time PCR assays targeting E. albertii-specific gene (EA-rtPCR) was designed. With 74 strains, including 43 E. albertiistrains and several of its close relatives, EA-rtPCR specifically amplified E. albertii; therefore, the sensitivity of EA-rtPCR was then evaluated. The detection limits were 2.8 and 2.0–3.2 log colony-forming unit (CFU)/mL for E. albertiiculture and enriched chicken culture inoculated with the pathogen, respectively. In addition, E. albertiiwas detected from 25 g of chicken meat inoculated with 0.1 log CFU of the pathogen by EA-rtPCR. The detection of E. albertiifrom chicken meat by EA-rtPCR was also evaluated by comparing with the nested-PCR assay, and 28 retail chicken meat and 193 dissected body parts from 21 chicken carcass were tested. One and three chicken meat were positive in the nested-PCR assay and EA-rtPCR, respectively. Fourteen carcasses had at least one body part that was positive for EA-rtPCR, and 36 and 48 samples were positive for the nested-PCR assay and EA-rtPCR, respectively. A total of 37 strains of E. albertiiwere isolated from seven PCR-positive samples obtained from six chicken carcass. All E. albertiiisolates harbored eaegene, and were classified as E. albertiiO-genotype (EAOg)3 or EAOg4 by EAO-genotyping. The EA-rtPCR developed in this study has potential to improve E. albertiidetection in food and advance research on E. albertiiinfection. |
| Databáze: |
Supplemental Index |
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