| Autor: |
Rangu, Shyam Sunder, Hajare, Sachin N., Sarkar, Sourav, Bhowmick, Sandip, Misra, Chitra S., Bindal, Gargi, Singh, K. K., Mukhopadhyaya, Rita, Rath, Devashish |
| Zdroj: |
Biomass Conversion and Biorefinery; 20240101, Issue: Preprints p1-11, 11p |
| Abstrakt: |
Use of tributyl phosphate (TBP) in industry leads to generation of large volume of TBP-containing wastes. TBP causes toxicity to marine life in aquatic habitats. Animal studies have shown toxicity in rats and rabbits upon ingestion of TBP. Sphingobiumsp. RSMS (RSMS) was reported to efficiently utilise TBP (30 mM in 3 days) as the sole source of carbon and phosphorous at laboratory scale. Here, we develop processes for the step-wise scale-up of TBP biodegradation utilizing RSMS strain. At 30 L scale, under optimised conditions, we could achieve almost complete mineralisation (28 mM in 3 days). Computational fluid dynamics modelling was used to visualise the flow patterns and to identify the optimum values of operating parameters for scale-up to 205 L. To develop an economical process, the 205 L scale-up was standardized under non-sterile conditions, ambient temperature and low initial culture density. Under these conditions, the strain could efficiently utilise TBP for growth and achieve a total of 21 mM TBP degradation over 15 days, which is approximately 70% of the TBP biodegradation observed at laboratory scale. |
| Databáze: |
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