Steinernema africanumn. sp. (Rhabditida, Steinernematidae), a New Entomopathogenic Nematode Species Isolated in the Republic of Rwanda

Autor: Machado, Ricardo A. R., Bhat, Aashaq Hussain, Abolafia, Joaquín, Shokoohi, Ebrahim, Fallet, Patrick, Turlings, Ted C. J., Tarasco, Eustachio, Půža, Vladimír, Kajuga, Joelle, Yan, Xun, Toepfer, Stefan
Zdroj: Journal of Nematology; September 2022, Vol. 54 Issue: 1
Abstrakt: Alternatives to hazardous insecticides are urgently needed for an environmentally friendly and effective management of insect pests. One such option is the use of entomopathogenic nematodes (EPN). To increase the availability of EPN with potential for biocontrol, we surveyed agricultural soils in the Republic of Rwanda and collected two Steinernemaisolates. Initial molecular characterization showed that they represent a new species, for which we propose the name S. africanumn. sp. To describe this new species, we reconstructed phylogenetic relationships, calculated sequence similarity scores, characterized the nematodes at the morphological level, conducted crossing experiments, and isolated and characterized their symbiotic bacteria. At the molecular level, S. africanumn. sp. is closely related to S. litoraleand S. weiseri. At the morphological level, S. africanumn. sp. differs from closely related species by the position of the nerve ring and also because the stoma and pharynx region is longer. The first-generation males have ventrally curved spicules with lanceolate manubrium and fusiform gubernaculum and the second-generation males have rounded manubrium and anteriorly hook-like gubernaculum. Steinernema africanumn. sp. does not mate or produce fertile progeny with any of the closely related species.
Databáze: Supplemental Index