| Autor: |
Sato, Naofumi, Shirakawa, Kamon, Kakihara, Youko, Mochizuki, Hiroshi, Kanamori, Tosinori |
| Zdroj: |
Analytical Sciences; December 1996, Vol. 12 Issue: 6 p853-858, 6p |
| Abstrakt: |
A highly sensitive chemiluminescence assay system for human chorionic gonadotropin (hCG) and thyroid stimulating hormone (TSH) was developed using a newly synthesized acridinium ester (AE) with a binding substitutional group at the N-10 position and having excellent chemiluminescence and stability. AE-labeled antibodies with an excellent chemiluminescence intensity were prepared after studying the labeling of anti-hCG antibodies with AE under different conditions. By studying the purification conditions for AE-labeled antibodies we produced AE-labeled antibodies having a low background value when assayed. When the detection limit is defined as the signal value+3SD at a concentration of zero for each substance measured, the detection limit for hCG is 0.01 IU/l and that for TSH is 0.001 μIU/ ml, demonstrating extremely high sensitivity. The labeled antibody’s stability is also excellent. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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