| Autor: |
Marekov, Ilko, Tarandjiiska, Roumyana, Panayotova, Svetlana, Nikolova, Nelly |
| Zdroj: |
Journal of Planar Chromatography - Modern TLC; October 2001, Vol. 14 Issue: 5 p384-390, 7p |
| Abstrakt: |
A stand-alone argentation TLC (Ag-TLC) procedure has been established for rapid estimation of the authenticity and densitometric quantitation of fatty acid groups in milk fats. The applicability of methyl (ME) and isopropyl (IPE) ester derivatives for accurate short-chain fatty acid analysis by Ag-TLC and GLC was compared and a correction coefficient compensating for loss of C4–C10saturated fatty acids has been proposed. To check its accuracy and reproducibility the method was applied to model mixtures and to real margarine and butter samples. Low levels of trans fatty acids can be unequivocally detected and amounts down to 0.2% can be measured precisely. The method gives a good estimate of the diversity of isomeric fatty acids in the samples and when precautions are taken to avoid overloading, it provides reliable results with good accuracy. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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