| Autor: |
Sangeet, Satyam, Pawar, Sarika, Nawani, Neelu, Junnarkar, Manisha, Gaikwad, Swapnil |
| Zdroj: |
3 Biotech; November 2022, Vol. 12 Issue: 11 |
| Abstrakt: |
In this study we aim to investigate the computational docking approach of biofabricated silver nanoparticles against P. aeruginosavirulent exoenzymes, such as ExoS and ExoY. Therefore, the synthesis and characterization of biofabricated silver nanoparticles using Piper betleleaves (Pb-AgNPs) were carried out. The surface topology and functional group attachment on the surface of Pb-AgNPs were analyzed using UV–visible spectroscopy, Scanning Electron Microscopy, Fourier Transformed Infrared Spectroscopy (FTIR), and X-Ray Diffraction. The FTIR analysis revealed that the synthesized silver nanoparticles were capped with P. betlephytochemicals importantly Eugenol and Hydroxychavicol. These are the major bioactive compounds present in P. betleleaves; therefore, computational docking of Eugenol-conjugated AgNPs (PbEu-AgNPs) and Hydroxychavicol-conjugated AgNPs (PbHy-AgNPs) against ExoS and ExoY was performed. The active residues of PbEu-AgNPs and PbHy-AgNPs interacted with the active site of ExoS and ExoY exoenzymes. Biofabricated AgNP-mediated inhibition of these virulent exoenzymes blocked the adverse effect of P. aeruginosaon the host cell. The computational analysis provides new approach into the design of biofabricated AgNPs as promising anti-infective nanomedicine agents. |
| Databáze: |
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