| Autor: |
Shumyantseva, V. V., Kuzikov, A. V., Masamrekh, R. A., Filippova, T. A., Koroleva, P. I., Agafonova, L. E., Bulko, T. V., Archakov, A. I. |
| Zdroj: |
BioNanoScience; December 2022, Vol. 12 Issue: 4 p1341-1355, 15p |
| Abstrakt: |
Enzymes are powerful and effective biological catalysts demanded in various areas of human activity, especially in medicinal area and as modem diagnostic tools. Enzymes-based electrode sensors (2D format) and nanopore sensors (3D format) represent modern, high-tech areas of bionanotechnology and bionanoscience. A comparative analysis of the catalytic activity of enzymes on flat electrode as a 2D sensor, on an electrode functionalized with a nanoporous material based on indium tin oxide as a transition from 2 to 3D model, and in a biological nanopore as a 3D sensor was carried out. Analysis of electrochemical properties of substrate or product/metabolite for the assessment of catalytic activity of enzymes was described. Comparative kinetic parameters of enzymes immobilized on an electrode and in a nanopore as sensors are given. The authors present the prospects of both electrochemical studies of enzymes using electrode sensor and studies of enzymes or substrates confined in a nanopore sensor. |
| Databáze: |
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