| Abstrakt: |
The Pilumnus spinulosusspecies belonging to the Pilumnusgenus was obtained with the sampling study carried out from the estuary of the Black Sea coast in 2017–2020. Temperature, salinity, dissolved oxygen, pH, TOC parameters were measured seasonally at the stations where the target species was encountered. Habitat characteristics were recorded. Samples were collected from 0–10 m depth by beam trawl and diving.The first fixation of the collected samples with 96% alcohol was made in the field. The individuals were examined under the Leica brand stereomicroscope and species identification was made. In addition, we sequenced the COI from 4 individuals belonging to genus Pilumnusfrom Black Sea coast of Türkiye. Approximately, 645 bp were amplified from the COI sequences. To identify Pilumnusindividuals, the sequences were comparised in GenBank BLAST. Our sequences matched to the Pilumnus spinulosusfrom Bulgaria with a similarity ratio of mean 99.38%. With this study, the distribution and environmental demands of the P. spinulosusspecies, which were not previously detected in the Black Sea coasts of Türkiye, were determined. |
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