| Autor: |
Srivastava, Akanksha, Misra, Ankita, Chaudhary, Mridul Kant, Shukla, Pushpendra Kumar, Kumar, Manish, Srivastava, Sharad |
| Zdroj: |
Proceedings of the National Academy of Sciences, India Section B; March 2023, Vol. 93 Issue: 1 p97-105, 9p |
| Abstrakt: |
M. dioicaRoxb. ex Willd. (Family: Cucurbitaceae) widely referred to as wild bitter gourd (Janglee karela), is well known plant used in Indian system of traditional medicine for the treatment of fever, diabetes, leprosy and snake bites. In this study, pharmacognostical standardization, proximate and pharmacological analysis was performed to validate its traditional claims as a food supplement. A validated method developed for HPTLC to determine the amount of caffeic acid in fruits. The chromatogram of the marker compound was developed using the tertiary mobile phase (Toluene: Ethyl acetate: Formic acid) ratio (6:4:0.5 v/v/v). Caffeic acid was detected at an Rf value 0.44 ± 0.04 and densiometric scanning was performed at 270 nm. The Caffeic acid content within the plant was found to be 0.0034% on dry weight basis. This method is highly accurate, linear and precise with respect to guidelines provided by ICH. The pharmacognostical parameters viz. moisture content, ash and extractive values of samples was found to be within the limits of standard. The phytochemical evaluation of metabolites through spectroscopy reveals the presence of flavonoid, phenolics, starch, sugar and tannin. Among them the highest value in starch estimation. The Pharmacological activities such as (Anti-diabetic, Anti-inflammatory & Anti-oxidant activity) were also analyzed. The findings showed that M.dioicahave most potential and is useful for future standardization, quality control and validation of plant material for final product in herbal drug industry. Furthermore, the results show that the fruits of M.dioicaare suitable for rich source of nutrients also as proteins, carbohydrates and lipids. |
| Databáze: |
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