| Autor: |
Zaane, D V, Gielkens, A L, Hesselink, W G, Bloemers, H P |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; May 1977, Vol. 74 Issue: 5 p1855-1859, 5p |
| Abstrakt: |
Polyadenylylated mRNA isolated from cells infected with Rauscher murine leukemia virus was fractionated by centrifugation in in a denaturing sucrose gradient into different sizes. Each RNA fraction was injected into oocytes of Xenopus laevis and the virus-specific products were analyzed by immunoprecipitation with polyvalent and monospecific antisera against polypeptides of Rauscher murine leukemia virus, and then by gel electrophoresis and scintillation autoradiography. It was shown that a 35S mRNA species directs the synthesis of a precursor of the internal or group-specific antigens of the virion (the gag-gene products). A 22S mRNA species directs the synthesis of two viral envelope polypeptides and their precursor polypeptide (env-gene products). The results indicate that the gag- and env-related polypeptides of Rauscher murine leukemia virus are synthesized uncoordinately and provide evidence for open and closed cistrons on the virus-specific mRNAs. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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