| Autor: |
Lacatena, R M, Cellini, A, Scavizzi, F, Tocchini-Valentini, G P |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; October 1994, Vol. 91 Issue: 22 p10521-10525, 5p |
| Abstrakt: |
We have investigated the topology of the human beta 2-adrenergic receptor expressed in Escherichia coli, using the genetic method described by Beckwith and coworkers. We found that fusions with alkaline phosphatase beyond a certain point on the human beta 2-adrenergic receptor sequence were assembled into the bacterial membrane with the same topology as the human beta 2-adrenergic receptor in the mammalian membrane. The pattern that might have been expected on the basis of the topology of the human beta 2-adrenergic receptor in mammalian membranes was not reflected in the levels of alkaline phosphatase activity of the fusions occurring between the N-terminal region and positions close to the second external domain. Our data suggest that the correct positioning of the N terminus of the receptor depends on the presence of its C-terminal portions. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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