| Autor: |
Merritt, S C, Tschudi, C, Konigsberg, W H, Richards, F F |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; March 1983, Vol. 80 Issue: 6 p1536-1540, 5p |
| Abstrakt: |
African trypanosomes change their antigenicity by successively expressing different members of a group of related but highly diverse proteins, the variant surface glycoproteins (VSGs). We describe a synthetic oligonucleotide that can prime specifically reverse transcription of VSG mRNA out of total trypanosome poly(A)+ RNA. The specificity of this priming was verified by cDNA sequence analysis of the transcription products and by the demonstration of variant-specific hybridization of the individual cDNAs to cellular RNA. The oligonucleotide primer also was used as a probe for the conserved sequence found on these VSG mRNAs in trypanosome genomic DNA libraries. A large number of primer-positive clones were detected in a Trypanosoma gambiense genomic library, but very few positive signals were found in a library of Trypanosoma congolense genomic DNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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