| Autor: |
Zebedee, S L, Barbas, C F, Hom, Y L, Caothien, R H, Graff, R, DeGraw, J, Pyati, J, LaPolla, R, Burton, D R, Lerner, R A |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; April 1992, Vol. 89 Issue: 8 p3175-3179, 5p |
| Abstrakt: |
Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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