| Autor: |
Burlingame, R P, Obukowicz, M G, Lynn, D L, Howe, M M |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; August 1986, Vol. 83 Issue: 16 p6012-6016, 5p |
| Abstrakt: |
Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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