| Autor: |
von Kalm, L, Weaver, J, DeMarco, J, MacIntyre, R J, Sullivan, D T |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; July 1989, Vol. 86 Issue: 13 p5020-5024, 5p |
| Abstrakt: |
In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis of these isoforms, we have sequenced a 5.8-kilobase region of the Drosophila genome that contains the entire Gpdh locus. Primer-extension and RNase protection assays show that the gene consists of eight exons and has a single transcription-start point. RNase protection mapping and comparison of the genomic sequence from three different cDNA clones reveal that three protein isoforms of glycerol-3-phosphate dehydrogenase are produced by alternative processing of 3' exons. Two of the isoforms differ from the third by the addition of either three or ten amino acids to their C-terminal ends. Transcripts corresponding to two of the isoforms are expressed during both larval and adult stages, while the third isoform is produced only in adults. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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