| Autor: |
Hedley, M L, Amrein, H, Maniatis, T |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; December 1995, Vol. 92 Issue: 25 p11524-11528, 5p |
| Abstrakt: |
We have identified an amino acid sequence in the Drosophila Transformer (Tra) protein that is capable of directing a heterologous protein to nuclear speckles, regions of the nucleus previously shown to contain high concentrations of spliceosomal small nuclear RNAs and splicing factors. This sequence contains a nucleoplasmin-like bipartite nuclear localization signal (NLS) and a repeating arginine/serine (RS) dipeptide sequence adjacent to a short stretch of basic amino acids. Sequence comparisons from a number of other splicing factors that colocalize to nuclear speckles reveal the presence of one or more copies of this motif. We propose a two-step subnuclear localization mechanism for splicing factors. The first step is transport across the nuclear envelope via the nucleoplasmin-like NLS, while the second step is association with components in the speckled domain via the RS dipeptide sequence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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