| Abstrakt: |
In a study of juvenile hormone-regulated gene expression, we isolated an anonymous cDNA representing a message that was strongly suppressed by juvenile hormone in the fat body of the cockroach Blaberus discoidalis. The protein deduced from the cDNA sequence showed compelling resemblance in sequence to the transferrins, a superfamily of internally duplicated, 80-kDa iron-binding/transport proteins characterized from several vertebrates and, to date, one insect (the tobacco hornworm, Manduca sexta). We isolated a 78-kDa protein from cockroach hemolymph, verified its congruence with the cloned cDNA, and found that it did bind iron. The cockroach protein is a member of the transferrin superfamily based on several features, including 32-46% amino acid positional identity with transferrins whose sequences are known, internal homology, positioning of cysteine residues, and iron binding. Whereas the previously characterized insect transferrin binds one atom of iron per protein molecule, B. discoidalis transferrin binds two iron atoms as do the vertebrate transferrins. The diferric property of cockroach transferrin is consistent with presence of two sets of residues positioned appropriately for iron binding. Juvenile hormone suppressed transferrin mRNA levels drastically in the adult female cockroach. |
