| Autor: |
Ross, A H, Grob, P, Bothwell, M, Elder, D E, Ernst, C S, Marano, N, Ghrist, B F, Slemp, C C, Herlyn, M, Atkinson, B |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; November 1984, Vol. 81 Issue: 21 p6681-6685, 5p |
| Abstrakt: |
The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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