| Autor: |
Zabel, B U, Naylor, S L, Sakaguchi, A Y, Bell, G I, Shows, T B |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; November 1983, Vol. 80 Issue: 22 p6932-6936, 5p |
| Abstrakt: |
The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism: amylase gene (AMY) to 1p21; proopiomelanocortin gene (POMC) to 2p23, somatostatin gene (SST) to 3q28, and a single copy DNA segment (D3S1) to 3q12. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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