| Autor: |
Garman, R D, Ko, J L, Vulpe, C D, Raulet, D H |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; June 1986, Vol. 83 Issue: 11 p3987-3991, 5p |
| Abstrakt: |
We have examined T-cell receptor alpha- and beta-chain variable (V) region gene usage in T-cell populations predicted to have different major histocompatibility complex-restriction specificities. Using a sensitive ribonuclease protection assay to measure T-cell receptor mRNA levels, we found no striking differences in the usage of three V alpha genes and three V beta genes in T-cell populations from three congeneic H-2-disparate strains of mice and between the mutually exclusive Ly2+ L3T4- and Ly2- L3T4+ T-cell subpopulations. These results suggest that major histocompatibility complex restriction cannot be explained by the differential usage of nonoverlapping V alpha or V beta gene pools. In contrast, striking but unpredictable differences were seen in V gene usage in populations of T cells selected by activation with particular alloantigens. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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