| Autor: |
Thomas, K A, Rios-Candelore, M, Fitzpatrick, S |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; January 1984, Vol. 81 Issue: 2 p357-361, 5p |
| Abstrakt: |
Acidic brain fibroblast growth factor has been purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Two microheterogeneous forms of the molecule are obtained with apparent molecular masses of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at about 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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