| Autor: |
Semenza, G L, Nejfelt, M K, Chi, S M, Antonarakis, S E |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; July 1991, Vol. 88 Issue: 13 p5680-5684, 5p |
| Abstrakt: |
Human erythropoietin gene expression in liver and kidney is inducible by anemia or hypoxia. DNase I-hypersensitive sites were identified 3' to the human erythropoietin gene in liver nuclei. A 256-base-pair region of 3' flanking sequence was shown by DNase I protection and electrophoretic mobility-shift assays to bind four or more different nuclear factors, at least two of which are induced by anemia in both liver and kidney, and the region functioned as a hypoxia-inducible enhancer in transient expression assays. These results provide insight into the molecular basis for the regulation of gene expression by a fundamental physiologic stimulus, hypoxia. |
| Databáze: |
Supplemental Index |
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